In this interview, Khalila Haque, who just completed her Bachelor’s in General Biology at Indian River State College, shares the exciting results obtained using Murphy’s Natural Hemp CBD/Curcumin tincture in treating HeLa cancer cells during lab research.
Me: Good Morning Khalila, thanks so much for doing this interview with me, I know you’re buried in starting GRE studies and wrapping up school at the same time. The research from your Cell Biology and Physiology Lab, done with HeLa cancer cells and Murphy’s Natural Hemp CBD/Curcumin is incredibly amazing. I know you were excited to see the results and share it with us. Would you walk me through the steps?
Khalila: Sure, first let me add that I was part of a team, Myron Clemons, myself, and another student. Our hypothesis was that the “Application of CBD/Curcumin would inhibit the growth of HeLa cell culture because of CBD’s anti-proliferative effects on cancer types, and curcumin’s tumorigenesis pathway suppression.”
We followed the HeLa cell protocol prior to treatment, sterilized Murphy’s CBD/Curcumin complex and stored until needed for application, and then began treating each row [pictured below] with different concentrations of the sterilized complex.
So, you’ll see in the picture [below] several columns, each well contained roughly 2 ml of HeLa cell culture, mixed with bovine serum for enrichment. We filter sterilized the Murphy’s Natural Hemp CBD/curcumin complex before treatment. The first column was our control, the second column was treated with 5 ul of the CBD/Curcumin complex, the third with 10 ul of the complex, and the fourth with 20 ul of the complex. ul stands for microliter.
Me: And for our readers, I’m going to stop for just a moment to clarify what HeLa cells are. According to the John Hopkins Medicine website:
Among the important scientific discoveries of the last century was the first immortal human cell line known as “HeLa” — a remarkably durable and prolific line of cells obtained during the treatment of Henrietta’s cancer by Johns Hopkins researcher Dr. George Gey in 1951.
Although these were the first cells that could be easily shared and multiplied in a lab setting, Johns Hopkins has never sold or profited from the discovery or distribution of HeLa cells and does not own the rights to the HeLa cell line. Rather, Johns Hopkins offered HeLa cells freely and widely for scientific research. (1)
I found a little more detail about these “immortal cells.” This is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951 from Henrietta Lacks, a patient who died of cancer on October 4, 1951. (2)
Me: Continuing on, Khalila, tell me about this series of slides.
Khalila: Sure, you’ll notice in the images that follow that the results showed visibly less HeLa cell growth, and inhibition with the 20 ul concentration. There was not much of a difference between the 5 ul and the 10 ul dosages. The images below provide more detail, much more clear, and appear in the order of “control,” then from lower to higher CBD/Curcumin concentrations applied.
The first image – control:
The second & third: you can see a bit of the tincture spots:
And in the last photo: you can see the difference in cell count:
Me: So the Murphy’s Natural Hemp CBD/Curcumin inhibited cell growth in the HeLa cancer cells?
Khalila: Yes, that’s what we concluded. The 20 ul dilution wells had visibly less cell growth in comparison to control, 5 ul and 10 ul wells.
For our project, we assume it may silence the Id-2 and Id-1 gene, which slows cancer development, or inhibits the epidermal growth factor. What’s awesome about this is that another group used CBD oil, but they had a hard time because it wasn’t water-soluble, [like Murphy’s Natural Hemp]. They eventually had similar results, but they had to use a ridiculously high concentration of the oil! So the Murphy’s Natural Hemp CBD/Curcumin is waaaay more efficient and effective.
Based on our results, continuous research should be considered on the properties of CBD and its inhibition of cancerous cell growth. Not only has CBD shown benefit by possibly inhibiting Id-1 and Id2 gene expression, according to other research we have done, it can also be used to relieve side effects from chemotherapy, such as nausea and vomiting. In addition, research on Curcumin has proven its anti-inflammatory activity. This in combination with CBD should be further tested as it may drastically reduce inflammatory factors that lead to cancer development (Shruti, 2018).
Me: This is exciting research you’ve shared with us. Is there anything else you’d like to add about your work? Has this project in any way influenced your opinion of CBD and it’s health benefits?
Khalila: Personally, this experiment backed up my initial opinion of CBD, and it’s possible anti-cancer properties. I have read studies that have come up with similar results as well. It would be interesting to see future research testing and its effects on specific cellular pathways.
For more information regarding the interview, or to purchase Murphy’s Natural Hemp products, go to http://www.murphysnaturalhemp.com.