Murphy’s Natural Hemp CBD/Curcumin used in a HeLa Cancer Cell Experiment

In this interview, Khalila Haque, who just completed her Bachelor’s in General Biology at Indian River State College,  shares the exciting results obtained using Murphy’s Natural Hemp CBD/Curcumin tincture in treating HeLa cancer cells during lab research.

Me:  Good Morning Khalila, thanks so much for doing this interview with me, I know you’re buried in starting GRE studies and wrapping up school at the same time.  The research from your Cell Biology and Physiology Lab, done with HeLa cancer cells and Murphy’s Natural Hemp CBD/Curcumin is incredibly amazing.  I know you were excited to see the results and share it with us.  Would you walk me through the steps?  

Khalila:  Sure,  first let me add that I was part of a team, Myron Clemons, myself, and another student.  Our hypothesis was that the “Application of CBD/Curcumin would inhibit the growth of HeLa cell culture because of CBD’s anti-proliferative effects on cancer types, and curcumin’s tumorigenesis pathway suppression.”

We followed the HeLa cell protocol prior to treatment, sterilized Murphy’s CBD/Curcumin complex and stored until needed for application, and then began treating each row [pictured below] with different concentrations of the sterilized complex. 

So, you’ll see in the picture [below] several columns, each well contained roughly 2 ml of HeLa cell culture, mixed with bovine serum for enrichment.  We filter sterilized the Murphy’s Natural Hemp CBD/curcumin complex before treatment.  The first column was our control, the second column was treated with 5 ul of the CBD/Curcumin complex, the third with 10 ul of the complex, and the fourth with 20 ul of the complex.  ul stands for microliter.

Me:  And for our readers, I’m going to stop for just a moment to clarify what HeLa cells are.  According to the John Hopkins Medicine website:

Among the important scientific discoveries of the last century was the first immortal human cell line known as “HeLa”  — a remarkably durable and prolific line of cells obtained during the treatment of Henrietta’s cancer by Johns Hopkins researcher Dr. George Gey in 1951.

Although these were the first cells that could be easily shared and multiplied in a lab setting, Johns Hopkins has never sold or profited from the discovery or distribution of HeLa cells and does not own the rights to the HeLa cell line. Rather, Johns Hopkins offered HeLa cells freely and widely for scientific research. (1)

I found a little more detail about these “immortal cells.”  This is the oldest and most commonly used human cell line.  The line was derived from cervical cancer cells taken on February 8, 1951 from Henrietta Lacks, a patient who died of cancer on October 4, 1951. (2) 

Me:  Continuing on, Khalila, tell me about this series of slides.

Khalila:  Sure, you’ll notice in the images that follow that the results showed visibly less HeLa cell growth, and inhibition with the 20 ul concentration.  There was not much of a difference between the 5 ul and the 10 ul dosages.  The images below provide more detail, much more clear, and appear in the order of “control,” then from lower to higher CBD/Curcumin concentrations applied. 

The first image – control:

The second & third:  you can see a bit of the tincture spots:

And in the last photo: you can see the difference in cell count:

Me:  So the Murphy’s Natural Hemp CBD/Curcumin inhibited cell growth in the HeLa cancer cells?

Khalila:  Yes, that’s what we concluded.  The 20 ul dilution wells had visibly less cell growth in comparison to control, 5 ul and 10 ul wells. 

For our project, we assume it may silence the Id-2 and Id-1 gene, which slows cancer development, or inhibits the epidermal growth factor.  What’s awesome about this is that another group used CBD oil, but they had a hard time because it wasn’t water-soluble, [like Murphy’s Natural Hemp].  They eventually had similar results, but they had to use a ridiculously high concentration of the oil!  So the Murphy’s Natural Hemp CBD/Curcumin is waaaay more efficient and effective.

Based on our results, continuous research should be considered on the properties of CBD and its inhibition of cancerous cell growth.  Not only has CBD shown benefit by possibly inhibiting Id-1 and Id2 gene expression, according to other research we have done, it can also be used to relieve side effects from chemotherapy, such as nausea and vomiting.  In addition, research on Curcumin has proven its anti-inflammatory activity.  This in combination with CBD should be further tested as it may drastically reduce inflammatory factors that lead to cancer development (Shruti, 2018).

Me:  This is exciting research you’ve shared with us.  Is there anything else you’d like to add about your work?  Has this project in any way influenced your opinion of CBD and it’s health benefits?  

Khalila:  Personally, this experiment backed up my initial opinion of CBD, and it’s possible anti-cancer properties.  I have read studies that have come up with similar results as well.  It would be interesting to see future research testing and its effects on specific cellular pathways.

For more information regarding the interview, or to purchase Murphy’s Natural Hemp products, go to http://www.murphysnaturalhemp.com.

These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.
by Colleen Morgan
(1) https://www.hopkinsmedicine.org/henriettalacks/importance-of-hela-cells.html; (2)  https://www.ncbi.nlm.nih.gov/pubmed/19722756

CBD – Did you know that…

CBD cocktail by Justin Aikin

CBD is showing up everywhere!  There are CBD oils and creams, CBD infused foods, and now you can have it added to your favorite smoothie, coffee, or yes, your cocktail!  Cannabidiol, or CBD oil, is a type of cannabinoid that is found in the Hemp plant.  It does not have any psychoactive effects, so does not get the user “high.”  The cannabinoid has become increasingly popular over the last several years for treating patients with a range of medical conditions.  There’s a flood of information available about CBD and its benefits, but did you know that…

  • The medicinal properties of the cannabis plant have been known and respected for thousands of years, and not just for its flowers and stems.  The root was also used. (1)  A 2017 study speaks of the Roman historian, Pliny the Elder, “who wrote in his Natural Histories that ‘a decoction of the root in water relaxes contractions of the joints and cures gout and similar maladies.’ Throughout history, the hemp plant’s root has been recommended as a natural remedy for treating inflammation, joint pain, and gastrointestinal problems. (2)
  • CBD changes brainwaves.  Murphy’s Natural Hemp teamed with an FDA approved lab to run a case-study using the qEEG test in order to measure the effects our CBD/Curcumin product has on the brain.  During this particular case study, there appeared a consistent drop in wave Z-scores across all areas of the brain, especially in Delta, and Theta waves.  A reduction in Z-score is an indicator of a reduction in the symptoms associated with the particular brain wave.  The report shows a significant change in brainwave activity and lowered z-scores after using Murphy’s Natural Hemp CBD/Curcumin tincture.  Z-scores are indicators of activity, with zero being the norm or “mean,” the higher the z-score, the more noteworthy the health condition.  A lower or zero score is optimal.  There are a number of conditions that can be measured with this test, including but not limited to migraines, ADHD, Dyslexia, anxiety and depression, fibromyalgia, neuropathy, impaired memory, and more.
  • CBD appears to work with the body’s own natural pain management system called the Endocannabinoid System (ECS) that creates natural anti-inflammatory and painkilling effects.  The body’s ECS is made up of three components: cannabinoid receptors (the things that receive chemical signals outside the cell), endocannabinoids (small molecules that activate cannabinoid receptors), and metabolic enzymes that break down endocannabinoids after they are used.  “All vertebrate animals [naturally] express at least two cannabinoid receptors.  The CB1 receptor principally functions in the nervous system but is expressed in many cells throughout the body.  CB2 receptors are primarily associated with cells governing immune function.”(3)  Researchers believe that CBD compounds may positively interact with these receptors.  Individuals living with Fibromyalgia may find CBD to be helpful in managing symptoms.
  • Industrial Hemp CBD oil is legal.  With the passing of the 2018 Farm Bill, Hemp has exploded in growth, production, and consumer use.  The farm bill did several things.  It provided subsidies for American farmers, reinforced locally grown foods, expanded trade opportunities, kept SNAP in place, and legalized the production of the hemp plant.  However, the natural chemical compound that comes from the hemp or cannabis sativa plant that is used in products and sold on the market must contain less than 0.3% delta-9-tetrahydrocannabinol (THC), the euphorigenic component of cannabis.  Be an informed consumer!
  • Your pet has the same endocannabinoid system you do.  CBD may be helpful for managing your pet’s anxiety during times of stress, or when they are suffering from pain and inflammation due to arthritis. (4)
  • The contents listed on the label of your CBD product may not be accurate!  Over 2015 through 2017, the U.S. Food and Drug Administration sent out warning letters to several companies that market products containing CBD.  The FDA tested the chemical content of the CBD compounds in some of these products and found that many were mislabeled.  They, in fact, did not contain the amount of CBD printed on the labels.  Additionally, they’ve recently sent out more letters, and according to their April 2, 2019 press release have scheduled, “a public hearing on May 31, as well as a broader opportunity for written public comment, for stakeholders to share their experiences and challenges with these products, including information and views related to product safety.” (5)  Choose a CBD company you can trust!
  • Water-soluble means the product is highly capable of dissolving in water.  It also means better absorption in the body!  Oil and water don’t mix.  One of the main challenges with CBD is guaranteeing the bioavailability of the active ingredients, especially when they have limited gastrointestinal permeability.  To improve aqueous solubility, and thus the therapeutic effectiveness of these compounds, Murphy’s Natural Hemp products are designed using pharmaceutical technologies to increase bioavailability.  See for yourself!

Where can you purchase Murphy’s Natural Hemp CBD Products? 

For more information, or to purchase Murphy’s Natural Hemp products, go to http://www.murphysnaturalhemp.com.

These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.
(1) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628559/; (2) https://scholar.google.com/scholar?q=Pliny(the+Elder)+.++The+natural+history+of+Pliny,+Volume+4;(3) British Journal of Pharmacology report: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4301686; (4) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065210/; (5)https://www.fda.gov/NewsEvents/Newsroom/PressAnnouncements/ucm635048.htm

CBD & the Human Body

Looking for natural alternatives

CBD has hit the market hard since the passing of the 2018 Farm Bill, which was great news for those of us looking for natural alternatives to prescription medications or over-the-counter remedies.  There has been a reported explosion in growth, production, selection of products, and consumer demand.  CBD is largely known for anxiety and pain relief, as well as muscle-relaxing properties, however, studies suggest this plant-derived natural remedy may also have additional health benefits.

How CBD words in the Human Body Clear graphic

Anxiety relief and more

With consumer use rapidly growing, research and clinical studies are also on the rise.  A recently published study on CBD for anxiety concluded that:

“Current understanding of the physiology and neurologic pathways points to a benefit with anxiety-related issues. The results of our clinical report support the existing scientific evidence. In our study, we saw no evidence of a safety issue that would limit future studies. In this evaluation, CBD appears to be better tolerated than routine psychiatric medications. Furthermore, CBD displays promise as a tool for reducing anxiety in clinical populations…”  (January 2019)

Additional trials and clinical studies were suggested to further confirm findings.  Still, this study echoes past findings from research conducted and published over many years, some as far back as 2001. (1) (2)  Other research indicates CBD use as beneficial for reducing inflammation, treatment for pain, combating arthritis, treating sleep disorders and more.

A very interesting study published by Pisanti, Malfitano, Ciaglia, and Lambert in February 2017, and later included in a World Health Organization (WHO) report November 2017, lists a number of diseases for which CBD may have therapeutic benefits.  The list includes Alzheimer’s, Parkinson’s, Multiple sclerosis, Huntington’s, Hypoxia-ischemia injury, Depression, Cancer, Nausea, Inflammatory bowel and Crohn’s, Cardiovascular and Diabetic complications. (3) (4)  As always, it’s important to talk with a physician before adding natural remedies to existing medications.

Murphy’s Natural Hemp Pairs CBD & Curcumin for increased health benefits

Murphy’s Natural Hemp pairs the benefits of CBD with the powerhouse spice, Curcumin.  Curcumin is receiving worldwide attention due to its multiple health benefits, which appear to act primarily through its anti-oxidant and anti-inflammatory mechanisms.  It is one of the main ingredients of turmeric powder, which is harvested from the turmeric plant by grinding up its root.  Curcumin has shown promising effects in patients with various pro-inflammatory diseases including cancer, cardiovascular disease, arthritis, ulcerative proctitis, Crohn’s disease, ulcerative colitis, irritable bowel disease, peptic ulcer, gastric ulcer, gastric inflammation, psoriasis, and lupus.

Water-Soluble Products

One of the main challenges with CBD and Curcumin is guaranteeing the bioavailability of the active ingredients, especially when they have limited gastrointestinal permeability.  To improve aqueous solubility, and thus the therapeutic effectiveness of these compounds, we researched countless formulation strategies, and were successful in finding a solution!  Murphy’s Natural Hemp products are designed with pharmaceutical technology that is typically used where circumstances demand increasing vital water-insoluble proteins for patients who are dealing with illnesses.

Unlike other CBD formulas, there is no nano complex in our solution, which only disperses small nano-size clusters of CBD molecules in an otherwise insoluble mixture. Eventually, all nano products separate, like oil and water… and, they show no improvement for increasing bioavailability.  And our products are made with 100% purified water, unlike oil-based CBD products offered by other companies.  This is what makes our products stand out among the rest.  99% of our product is absorbed by the body.  Only 10% of CBD is absorbed using the leading oil-based products.

Where can I buy Murphy’s Natural Hemp CBD Products? 

For more information, or to purchase Murphy’s Natural Hemp products, go to http://www.murphysnaturalhemp.com.

These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.
(1)Research and study information has been pulled from the US National Library of Medicine/National Institutes of Health at http://www.ncbi.nlm.nih.gov/pubmed. To search type in the cannabinoid name and health benefit.  (2) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326553/; (3) https://www.researchgate.net/publication/314080489; (4) https://www.who.int/medicines/access/controlled-substances/CannabidiolCriticalReview.pdf